Pure North Peptides Journal · Compendium
AOD-9604: HGH fragment 176–191 for lipolysis research
Published 2024-05-17 · Pure North Peptides Editorial · Canada
Short answer. AOD-9604 is the C-terminal 16-residue fragment of human growth hormone (residues 176–191) with an N-terminal tyrosine added during synthesis. It retains the lipolytic profile of full-length HGH without the somatotropic activity. AOD-9604 is not currently stocked at Pure North Peptides — see the current catalog for what we carry.
Why a 16-residue fragment matters
Native human growth hormone has two functionally separable activities: somatotropic (cell proliferation, IGF-1 induction via JAK2/STAT5) and lipolytic (adipocyte triglyceride hydrolysis, putatively via β3-adrenergic-receptor coupling). The C-terminal sequence 176–191 retains the latter without the former in published in-vitro adipocyte assays — useful for studying lipolysis pathways without confounding GH-receptor signaling.
Sequence and modification
The 16-residue active core is HGH 177–191; AOD-9604 adds an N-terminal Tyr (Y177) for synthesis-tag and analytical convenience. Total chain length 16 residues, mass 1817.10 Da.
Quality criteria
- RP-HPLC purity ≥ 99% at 220 nm.
- ESI-MS at 1817.10 Da ± 0.5 Da.
- Net peptide ≥ 80% by AAA.
- Endotoxin < 0.5 EU/mg for cell-line work.
In-vitro applications
3T3-L1 adipocyte differentiation models, primary adipocyte lipolysis assays (glycerol release readout), comparative pharmacology against full-length HGH. AOD-9604 is also used as a structural negative control for studies trying to dissect somatotropic versus lipolytic GH activity in receptor-knockout cell lines.
Reconstitution
Reconstitute with 1.0 mL bacteriostatic water for a 5 mg vial — concentration 5 mg/mL. The peptide is highly soluble at neutral pH and dissolves immediately. Stable 21 days at 2–8 °C after reconstitution.
AOD-9604 is not currently in the Pure North Peptides catalog. Email purenorthpeptides@proton.me if you would like to be notified if it is added in the future, or browse the current catalog →
Frequently asked questions
What does AOD-9604 stand for?
Anti-Obesity Drug 9604 — the development code from the original program characterizing the C-terminal HGH fragment for lipolytic effects.
Does AOD-9604 affect IGF-1 levels in research?
In published in-vitro models AOD-9604 lacks the somatotropic activity that drives IGF-1 induction by full-length HGH. Researchers report it as IGF-1-neutral in standard assays.
What is the molecular weight?
1817.10 Da. ESI-MS confirmation should fall within 0.5 Da on a competent COA.
Is AOD-9604 stable lyophilized?
Yes, in a desiccated, nitrogen-flushed vial it's stable for 24+ months.
Where to source AOD-9604 in Canada?
AOD-9604 is not currently stocked in the Pure North Peptides catalog. Email purenorthpeptides@proton.me to ask about future availability.
Disclaimer: All Pure North Peptides products are supplied for laboratory research use only. They are not approved by Health Canada as drugs. See our research-use declaration for full terms.