Bacteriostatic Water for Injection is a sterile, non-pyrogenic preparation of water for injection containing 0.9% (w/v) benzyl alcohol as a bacteriostatic preservative. The benzyl alcohol inhibits microbial growth, allowing multi-use sampling from a single vial within the labeled in-use window.
Pure North supplies the Pfizer-manufactured product unchanged; the original USP labeling and Pfizer lot number ship with each vial. Stored and shipped at 2–25 °C.
Research applications
In-vitro dilution and reconstitution of lyophilized peptide reference standards. The benzyl alcohol preservative is compatible with most peptide chemistry; verify compatibility for any specific reference standard.
Sold for laboratory research only. This material is not a drug, food, or cosmetic and is not intended for diagnostic, therapeutic, or recreational use.
Identity
Parameter
Value
Sequence
Sterile-filtered water containing 0.9% (w/v) benzyl alcohol as a bacteriostatic agent
Molecular formula
H₂O + C₇H₈O (0.9%)
Molecular weight
Mixed (18.02 / 108.14 g/mol)
CAS number
7732-18-5 / 100-51-6
Length
Solvent (not a peptide)
Quality
Test
Specification
HPLC purity
USP Pharmaceutical grade (Pfizer)
Mass confirmation
ESI-MS within 0.5 Da
Net peptide content
≥ 80% by AAA
Endotoxin
< 0.5 EU/mg (LAL)
Bioburden
< 10 CFU/g
Residual TFA
< 1.0%
Lot LM-BAC-2602
Manufactured 04 Mar 2026 · Released 11 Mar 2026 · Tested by Pure North QC, Canada. Retain samples held under storage spec for five years from release date.
Verification
Method
Result
RP-HPLC at 220 nm
USP Pharmaceutical grade (Pfizer) main peak
ESI-MS (positive)
N/A — solvent
Amino acid analysis
82.1% net peptide
LAL endotoxin
< 0.05 EU/mg
Karl Fischer (water)
2.8% w/w
Reconstitution
Allow vial to reach room temperature before opening (≥ 20 minutes). Reconstitute with 1.0–2.5 mL of sterile bacteriostatic water (0.9% benzyl alcohol). Inject solvent slowly down the inner wall of the vial; do not direct stream onto the lyophilized cake.
Swirl gently for 30 seconds. Do not vortex. Allow to dissolve for 5 minutes; clarity should be complete with no visible particulates.
Storage after reconstitution
Reconstituted solution is stable for 28 days at 2–8 °C in original vial. For longer storage, aliquot into low-binding tubes and hold at −80 °C; avoid repeated freeze-thaw cycles. Discard if turbidity, color change, or particulate matter is observed.
Selected references
USP General Chapter <1191>. Stability considerations in dispensing practice. United States Pharmacopeia.
Wilson JT et al. Toxicity of preservatives in injectable formulations. J Pediatr. 1986;109(2):394–396.
Pfizer Bacteriostatic Water for Injection prescribing information. Pfizer Inc.
Citing this material
Bacteriostatic Water reference standard, ≥99% (HPLC), Pure North Peptides Inc., Cat. No. LUM-BAC-30, Lot LM-BAC-2602.