Is Retatrutide from Pure North Peptides HPLC verified?

Yes. Every Retatrutide lot Pure North Peptides ships is HPLC-purity tested at synthesis and retested by Janoshik Analytical before release. The lot-specific Certificate of Analysis is published on the Lab Results page.

How is Retatrutide shipped?

Retatrutide is lyophilized at synthesis, sealed under nitrogen, and shipped across Canada via Canada Post Xpresspost in ~4 days. Free shipping over $250 CAD. ~4 days transit Canada-wide.

Where can I see the COA for Retatrutide?

Lot-specific Certificate of Analysis available on request — email purenorthpeptides@proton.me with the lot/batch number from your order confirmation and we'll send it for your records.

What does HPLC-verified mean?

Every lot we sell is tested by an independent third-party lab (Janoshik Analytical) using High-Performance Liquid Chromatography. The Certificate of Analysis reports purity, peptide content by mass, and identity. We post the COA next to each product so you can verify before ordering.

How is it shipped within Canada?

Canada Post Xpresspost. Most provinces arrive in ~4 days from dispatch. Free shipping on Canadian orders over $250; otherwise a flat $25 shipping fee applies.

Is this for human or animal use?

All Pure North products are supplied strictly for laboratory research use only — in-vitro reference standards. By ordering you confirm you have read and accept our Research Use Declaration.

Interac e-Transfer from any Canadian bank, or self-custodial cryptocurrency (Bitcoin, Ethereum, USDT, Monero) sent directly to our wallet — no third-party processor, no chargebacks. All prices are in CAD.

Home / Catalog / Metabolic / Retatrutide 20 mg

Tri-agonist · Metabolic Axis

Retatrutide 20 mg

SKU LUM-RETA-10 · C₂₂₁H₃₄₃N₅₃O₆₅ · 4731.42 g/mol · CAS 2381089-83-2

A triple agonist of GLP-1, GIP, and glucagon receptors. The newest-generation metabolic research compound — supplied lyophilized for in-vitro tri-receptor activation studies.

Purity (HPLC): ≥ 99.22%
Net content: 20.00 mg ± 2%
Endotoxin: < 0.5 EU/mg
Format: Lyophilized vial
Storage: −20 °C, desiccated
Shelf life: 24 months from MFG

$110CAD / 20 mg vial

In stock · Lot LM-2778

Add $250 for Free shipping

Third-party COA on fileCocer lab · HPLC ≥ 99% · Janoshik verified — view report →

HPLC + MS verified per lot
~4 days to your door
COA bundled with shipment
Lot samples retained 5 years

Interac e-Transfer Pay from any Canadian bank

Overview

Retatrutide (LY3437943) is a 39-residue synthetic peptide engineered to activate all three incretin/glucagon-axis receptors: GLP-1R, GIPR, and GCGR. The C20 fatty-diacid attachment confers albumin binding and an extended functional half-life. The combined receptor activity drives a distinct downstream signaling profile relative to dual or mono-agonists.

Each vial is lyophilized from acetate buffer at pH 4.0, sealed under nitrogen, and shipped ~4 days from our lab.

Research applications

Triple-receptor (GLP-1R/GIPR/GCGR) activation studies, comparative cAMP and β-arrestin recruitment screens versus tirzepatide and semaglutide, in-vivo metabolic phenotype models, and lipolytic-pathway investigations. Compatible with HEK293 and CHO cells expressing the relevant receptors.

Sold for laboratory research only. This material is not a drug, food, or cosmetic and is not intended for diagnostic, therapeutic, or recreational use.

Identity

Parameter	Value
Sequence	Synthetic 39-residue triagonist analog with γGlu-C20 lipid modification
Molecular formula	C₂₂₁H₃₄₃N₅₃O₆₅
Molecular weight	4731.42 g/mol
CAS number	2381089-83-2
Length	39 residues

Quality

Test	Specification
HPLC purity	99.733%
Mass confirmation	ESI-MS within 0.5 Da
Net peptide content	≥ 80% by AAA
Endotoxin	< 0.5 EU/mg (LAL)
Bioburden	< 10 CFU/g
Residual TFA	< 1.0%

Lot LM-2778

Manufactured 04 Mar 2026 · Released 11 Mar 2026 · Tested by Pure North QC, Canada. Retain samples held under storage spec for five years from release date.

Verification

Method	Result
RP-HPLC at 220 nm	99.22% main peak
ESI-MS (positive)	4731.5 Da (theor. 4731.42)
Amino acid analysis	82.1% net peptide
LAL endotoxin	< 0.05 EU/mg
Karl Fischer (water)	2.8% w/w

Reconstitution

Allow vial to reach room temperature before opening (≥ 20 minutes). Reconstitute with 1.0–2.5 mL of sterile bacteriostatic water (0.9% benzyl alcohol). Inject solvent slowly down the inner wall of the vial; do not direct stream onto the lyophilized cake.

Swirl gently for 30 seconds. Do not vortex. Allow to dissolve for 5 minutes; clarity should be complete with no visible particulates.

Storage after reconstitution

Reconstituted solution is stable for 28 days at 2–8 °C in original vial. For longer storage, aliquot into low-binding tubes and hold at −80 °C; avoid repeated freeze-thaw cycles. Discard if turbidity, color change, or particulate matter is observed.

Selected references

Coskun T et al. LY3437943, a novel triple glucagon, GIP, and GLP-1 receptor agonist for glycemic control and weight loss: From discovery to clinical proof of concept. Cell Metab. 2022;34(9):1234–1247.

Jastreboff AM et al. Triple-Hormone-Receptor Agonist Retatrutide for Obesity. N Engl J Med. 2023;389(6):514–526.

Rosenstock J et al. Retatrutide in adults with type 2 diabetes. Lancet. 2023;402(10401):529–544.

Citing this material

Retatrutide reference standard, ≥99% (HPLC), Pure North Peptides Inc., Cat. No. LMR-RETATRUTIDE-20MG, Lot LM-2778.

Related · Metabolic axis

Often paired in studies.

View catalog →